By J. Frank, M. Radermacher (auth.), James K. Koehler Ph. D. (eds.)
This quantity is a continuation of 2 earlier books on complex electron microscope ideas. the aim of this sequence has been to supply in intensity analyses of tools that are thought of to be on the cutting edge of electron microscopic study tactics with purposes within the organic sciences. The venture of the current quantity continues to be that of a resource publication for the study practitioner or complex scholar, in particular one already good versed in uncomplicated electron optical tools. it isn't intended to supply in troductory fabric, nor can this modest quantity wish to hide the total spectrum of complicated expertise now to be had in electron microscopy. long ago decade, pcs have chanced on their approach into many study laboratories due to the large elevate in computing energy and stor age on hand at a modest rate. The ultrastructural sector has additionally benefited from this growth in a few methods with the intention to be illustrated during this quantity. half the contributions talk about applied sciences that both without delay or not directly make wide use of machine methods.
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Extra info for Advanced Techniques in Biological Electron Microscopy III
FRANK and M. RADERMACHER Apart from its application in producing accurately defined tilt series, the goniometer may also be used to augment the "natural" tilt range of a particle, to establish interconversion between views of differently oriented particles (see VERSCHOOR et al. 1984), or to furnish a single micrograph of a tilted specimen grid containing particles with defined orientation but random azimuths (Sect. 4). Some of the technical aspects of tilting experiments in the electron microscope have been discussed by LANGE (1976).
The most exhaustive representation of the reconstructed volume is as a set of halftone images where each image represents an individual section (Fig. 20) (Contours at levels that are equivalent throughout the volume can be interpreted as traces of a closed curved surface). This representation has obvious shortcomings, because it is not conductive to the formation of a three-dimensional perception of the object. , density thresholds) for other, more easily comprehensible representations to be determined.
11. A section of the 30 S ribosomal subunit (VERSCHOOR et al. 1984) displayed at different levels of resolution. Panels on the left displayed without low-pass filtration. Panels on the right: low-pass filtration was applied to limit the resolution to the level permitted by formula (10) in the text. The section displayed without resolution limitation shows strong streak artifacts due to the discrete projection angles, which run into the vicinity of the particle. In the resolution-limited version, the streaks are still present, but they stay at the periphery of the section, no longer interfering with the subunit body.
Advanced Techniques in Biological Electron Microscopy III by J. Frank, M. Radermacher (auth.), James K. Koehler Ph. D. (eds.)