Verena Beier, Cora Mund, Jörg D. Hoheisel (auth.), Harald's Analytics of Protein–DNA Interactions PDF

By Verena Beier, Cora Mund, Jörg D. Hoheisel (auth.), Harald Seitz (eds.)

ISBN-10: 3540481478

ISBN-13: 9783540481478

ISBN-10: 3540481508

ISBN-13: 9783540481508

Analytics of Protein-DNA Interactions covers tendencies in sleek biotechnology. All points of this interdisciplinary expertise, the place wisdom, tools and services are required from chemistry, biochemistry, microbiology, genetics, chemical engineering and computing device technology, are taken care of. additional information in addition to the digital model is out there at springer.com.

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4a). Nonlinearity of the ln d(R) dt = f (t) plot suggests that the interaction cannot be analyzed using a 1 : 1 model or that other processes, such as mass transport (Sect. 3), interfere with DNA · PROTEIN complex formation. Besides being an important diagnostic plot, the ln d(R) dt = f (t) function can also be used for evaluation of the DNA · PROTEIN complex formation rate constant kon . The plot kobs vs C is linear (Eq. 2) and the slope of this function represents the kon rate constant (Fig. 4b).

Fischer · C. org) [56, 79, 80]. a Evidence for expression in squamous epithelial tissues or cultured epidermal keratinocytes. b Evidence for expression in non-epithelial tissues. c The minimal regulatory region required for proper expression of the gene in transgenic mice or transfected tissue culture.

1 – Req /Rmax ) For a successful analysis the response value at the equilibrium Req for each protein concentration must be known. This requires long protein–DNA contact times, which can be achieved by injecting protein solutions at a slow flow rate (mass transfer does not influence the Req value). The second important parameter for analysis is the response value at protein saturation Rmax . It can be difficult to reach, especially for negatively cooperative interaction. In this case the theoretical maximum binding value can be used: RDNA MWProtein Rmaxcal = n , (11) MWDNA where RDNA = amount of immobilized DNA (RU) MWDNA = molecular weight of DNA MWProtein = molecular weight of protein n = number of protein binding sites on DNA .

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Analytics of Protein–DNA Interactions by Verena Beier, Cora Mund, Jörg D. Hoheisel (auth.), Harald Seitz (eds.)


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