By R. E. Spier, J. B. Griffiths, W. Berthold
Most crucial for possible and wealthy animal telephone expertise is the success of winning items therefore developing gain for sufferers and credibility for the industrial charm of this particularly younger know-how. The papers awarded during this quantity handle the newest concerns and glance to destiny advancements within the fields of animal mobilephone expertise. very important issues thought of in those shows contain downstream processing and regulatory safeguard facets. The twelfth ESCAT assembly lawsuits proceed to supply a whole assessment of this crucial subject and may be a useful reference resource for these interested by the creation and use of animal cells
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Extra resources for Animal Cell Technology. Products of Today, Prospects for Tomorrow
Three of the cell lines were initial transfectant s (A, B and C) while the other three cell lines (AI , A2 and A3) were obtained from cell line A following gene amplification by increasing the concentratio n of the glutamine synthetas e inhibitor, methionine sulphoximine, in the medium. All six cell lines were adapted to suspensio n culture. In parallel, the cell lines were passage d in attached culture for the same number of generation s in the same medium. There were no indications of instability for any of these cell lines in either condition.
To confirm this indication, we demonstrate d (Figure 5) that the interaction could be blocked by an anti-VCAM monoclonal (IGll ) which inhibited similarly the binding of Jurkat cells to the protein produced either by S2LO-VCAM or Sf9 cells. 25 μg/ml, 40% inhibition is observedin both cases . Therefore, it seems that the epitope for I G l l is conserve d on both sources of protein. Figure 6. 15-5 μg/ml) Figure 5. Effect of I G l l antibody on activity of S2LO-VCAM and Sf9VCAM The legend indicates the expressio n system (S2LO, Sf9) and the X axis the antibody concentratio n 2200 r: i 2000 h 1800 1600 o1400 = 1200 i i 1 1S2LO .
Dissolved oxygen tension was maintained at 50% air saturation by direct oxygen gas sparging upon demand. Sf9 were infected by addition of a virus stock suspensio n so as to reach a multiplicity of infection of 5-10. S2LO-VCAM cells were induced by the addition of CUSO4 to a final concentratio n of 500μΜ Total cell density and viability were measure d in a hemocytometer , by the Trypan Blue exclusion method. Product characterisatio n Aliquots of fermentation samples were concentrate d by TCA precipitation and analysed by a modified Western Blot „procedure (a single step of peroxidase-couple d anti rabbit swine Immunoglobulins binding the ZZ tail of the VCAM fusion protein).
Animal Cell Technology. Products of Today, Prospects for Tomorrow by R. E. Spier, J. B. Griffiths, W. Berthold